Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 1. Specificity of anti-BMPRIA, anti-BMPRIB and anti-BMPRII antibodies. (A) Wells containing anti-BMPRIA antibody (IA-ab) show intense ELISA reaction only when coated by BMPRIA protein (IA-ag), but not BMPRIB protein (IB-ag). Wells containing anti-BMPRIB antibody (IB-ab) show intense ELISA reaction only when coated by BMPRIB protein (IB-ag), but not BMPRIA protein (IA-ag). Wells containing anti-BMPRII antibody show intense ELISA reaction only when coated by BMPRII protein (). (B) anti-BMPRIA, anti-BMPRIB and anti-BMPRII antibodies specifically recognize the corresponding proteins in a dose-dependent manner. (C–H) Photomicrographs of pre-absorption tests; control (C–E), pre-absorbed (F–H). Note that immunoreactivities in the layer V of the cortex are completely abolished by pre-absorption tests. Scale bar100 m for (C–H).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 2. BMPRIA, BMPRIB and BMPRII expressions in the rat whole brain. 12, hypoglossal nucleus; Amy, amygdala; AP, area postrema; cc, corpus callosum; Cer, cerebellum; CN, cerebellar nuclei; Cor, cerebral cortex; CPu, caudate putamen; DH, dorsal horn; Hi, hippocampus; IO, inferior olive; Pir, piriform cortex; VC, ventral cochlear nucleus, VH, ventral horn. Scale bar3 mm for (A–I); 2 mm for (J–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 3. BMPRIA, BMPRIB and BMPRII expressions in the olfactory bulb (A–I) and medial septal nucleus (J–L). EPl, external plexiform layer; Gl, glomerular layer; Gr, granular layer; MS, medial septal nucleus; ON, olfactory nerve layer; SE, subependymal layer. Scale bar 200 m for (A, D, G); 100 m for (B, C, E, F, H–L).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 4. BMPRIA, BMPRIB and BMPRII expressions in the cerebral cortex (A–D, F–I, K–N) and piriform cortex (E, J, O). I–VI, layers I–VI; Pir, piriform cortex. Scale bar200 m for (A, E, F, J, K, O); 100 m for (B–D, G–I, L–N).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 5. BMPRIA, BMPRIB and BMPRII expressions in the hippocampus. CA1-3, fields CA1-3 of Ammon’s horn; DG, dentate gyrus; Gr, granular layer; LM, stratum lacnosum-moleculare; Mo, stratum moleculare; Or, stratum oriens; Po, polymorphological layer; Py, stratum pyramidale; Ra, stratum radiatum. Scale bar 500 m for (A, D, G); 100 m for (B, E, H, J–L); 50 m for (C, F, I, M–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 6. BMPRIA, BMPRIB and BMPRII expressions in the basolateral amygdaloid nucleus (A–C), caudate putamen (D–F), globus pallidus (G–I) and corpus callosum (J–O). BL, basolateral amygdaloid nucleus; cc, corpus callosum; CPu, caudate putamen; dhc, dorsal hippocampal commissure; GP, globus pallidus. Scale bar100 m for (J–L); 50 m for (A–I, M–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 7. BMPRIA, BMPRIB and BMPRII expressions in the mediodorsal thalamic nucleus (A–C), reticular thalamic nucleus (D–F), supraoptic nucleus (G–I), red nucleus (J–L), substantia nigra, reticular part (M–O) and interpeduncular nucleus (P–R). IP, interpeduncular nucleus; MD, mediodorsal thalamic nucleus; RN, red nucleus; Rt, reticular thalamic nucleus; SNR, substantia nigra, reticular part; SO, supraoptic nucleus. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 8. BMPRIA, BMPRIB and BMPRII expressions in the fascial nerve nucleus (A–C), facial nerve (D–F), hypoglossal nucleus (G–I), ambiguus nucleus (J–L), nucleus of the solitary tract (M–O) and mesencephalic trigeminal nucleus (P–R). 7, facial nucleus; 7n, facial nerve; 12, hypoglossal nucleus; Amb, ambiguus nucleus; Me5, mesencephalic trigeminal nucleus; SOL, nucleus of the solitary tract. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 9. BMPRIA, BMPRIB and BMPRII expressions in the dorsal cochlear nucleus (A, C, E), ventral cochlear nucleus (B, D, F), lateral vestibular nucleus (G–I), vestibulo-cochlear nerve (J–L), inferior colliculus (M–O), olivary pretectal nucleus (P–R) and superior colliculus (S–U). 8n, vestibulo- cochlear nerve; DC, dorsal cochlear nucleus; IC, inferior colliculus; LVe, lateral vestibular nucleus; opt, optic tract nucleus; SC, superior colliculus; VC, ventral cochlear nucleus. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 10. BMPRIA, BMPRIB and BMPRII expressions in the locus ceruleus (A–C), pyramidal tract (D–F), inferior olive (G, J, M), cerebellar cortex (H, K, N), and cerebellar nuclei (I, L, O). CN, cerebellar nuclei; Gr, granular layer; IO, inferior olive; LC, locus ceruleus; Mol, molecular layer; Pur, Purkinje cell layer; Py, pyramidal tract. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 11. BMPRIA, BMPRIB and BMPRII expressions in the spinal cord (A–I), subventircular zone (J–L), choroidal plexus (M–O). Ch, choroidal plexus; DH, dorsal horn; VH, ventral horn; SVZ, subventricular zone; wm, white matter. Scale bar500 m for (A–C); 50 m for (D–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 12. Double-staining study showing that BMPRIA, BMPRIB and BMPRII positive cells in the corpus callosum (A, D, G) are also positive for GFAP (B, E, H) in a merged photomicrograph (C, F, I). Scale bar20 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques: Double Staining

Journal: Biology

Article Title: BMP-2-Driven Osteogenesis: A Comparative Analysis of Porcine BMSCs and ASCs and the Role of TGF-β and FGF Signaling.

doi: 10.3390/biology14060610

Figure Lengend Snippet: Figure 6. Receptor expressions of ALK2, ALK4, ALK6, ALK5, ALK7, BMPR-II in pASC. Grouped representation of the respective receptor expression in the course of osteogenic differentiation (OM +/−BMP-2). From day 19, there is a significant induction of ALK 2, ALK 6, and ALK 5 with the addition of BMP-2. BMPR-II expression in the OM group decreased in OM and tended to stay increased under BMP-2 supplementation from day 19, but was not considered significant (* p ≤0.05, ** p ≤0.01; n = 6, BMP-2 450 ng/mL).

Article Snippet: The respective conjugated antibodies were used for the expressions of ALK3 (Cat. No.: AF436), ALK 5 (Cat. No.: FAB5871), ALK6 (Cat. No.: FAB5051A), TGF-β2-RII (Cat. No.: FAB532P), ALK7 (Cat. No.: FAB77491A), ALK2 (Cat. No.: AF637), ALK4 (Cat. No.: MAB2221), and BMPR-II (Cat. No.: AF811) (by R&D Systems, Minneapolis, MN, USA), and the pASCs and pBMSCs were compared for their expressions of the specific surface antigens CD45 (Cat. No.: MCA1568GA, BioRad, Hercules, CA, USA), HLA-DR (human leukocyte antigen–antigen D-related surface molecule) (Cat. No.: MCA2314F, Bio-Rad, Hercules, CA, USA), CD29 (Cat. No.: 561,496, BD Pharmingen, Franklin Lakes, NJ, USA), CD79alpha (Bio-Rad, Cat. No.: MCA2538GA), CD14 (Cat. No.: MCA1568GA, Bio-Rad, Hercules, CA, USA), CD31 (Cat. No.: AF3387, R&D Systems, Minneapolis, MN, USA), CD105 (Cat. No.: NB110-58718APC, Novus Biologicals, Minneapolis, MN, USA), CD26 (, Cat. No.: NB600-552APC, Novus Biologicals, Minneapolis, MN, USA), CD73 (, Cat. No.: AF4488, R&D Systems, Minneapolis, MN, USA), CD90 (Cat. No.: 559,869, BD Pharmingen, Franklin Lakes, NJ, USA), CD34 (Cat. No.: 81289, abcam, Cambridge, UK), and CD44 (Cat. No.: 5531, BD Pharmingen, Franklin Lakes, NJ, USA).

Techniques: Expressing